文章信息/Info

Title:

Antimicrobial Surface Functionalization of Poly(arylene ether nitrile)s Containing Phthalazinone Moieties with Polypeptides

作者:

劉文韜1; 柳承德1; 2; 劉程1; 2; 3; 王錦艷1; 2; 3; 蹇錫高1; 2; 3

（1．大連理工大學(xué)化工學(xué)院高分子材料系，遼寧 大連 116024）（2．精細(xì)化工國(guó)家重點(diǎn)實(shí)驗(yàn)室，遼寧 大連 116024）（3．遼寧省高性能樹脂工程技術(shù)研究中心，遼寧 大連 116024）

Author(s):

LIU Wentao1;  LIU Chengde1; 2;  LIU Cheng1; 2; 3;  WANG Jinyan1; 2; 3;  JIAN Xigao1; 2; 3

(1.Department of Polymer Science and Materials，Dalian University of Technology，Dalian 116024，China)(2.State Key Laboratory of Fine Chemicals，Dalian University of Technology，Dalian 116024，China)(3.Liaoning Province Engineering Research Centre of High Performance Resins，Dalian University of Technology，Dalian 116024，China)

關(guān)鍵詞:

雜萘聯(lián)苯聚芳醚腈酮; 陽離子多肽; 表面改性; 細(xì)胞相容性; 抗菌活性

Keywords:

PPENK;  cationic polypeptide;  surface modification;  cytocompatibility;  antimicrobial activity

DOI:

10.7502/j.issn.1674-3962.201906029

文獻(xiàn)標(biāo)志碼:

A

摘要:

人工生物材料容易引起各種各樣的細(xì)菌感染。抗菌多肽因其優(yōu)異的抗菌性能及不易產(chǎn)生耐藥性細(xì)菌等優(yōu)點(diǎn)，被應(yīng)用于表面改性生物材料。雜萘聯(lián)苯聚芳醚腈酮（PPENK）因具有優(yōu)良的力學(xué)性能及易于消毒等特點(diǎn)，有望作為聚芳醚類生物材料。采用表面化學(xué)改性的方法在PPENK表面固定陽離子抗菌多肽，可賦予其抗菌性能，拓展雜萘聯(lián)苯聚芳醚材料在生物醫(yī)用領(lǐng)域的應(yīng)用范圍。采用伯胺引發(fā)氨基酸環(huán)內(nèi)酸酐開環(huán)聚合的方法，合成結(jié)構(gòu)不同的兩種多肽，然后利用點(diǎn)擊化學(xué)的原理，將多肽固定到PPENK表面。通過核磁共振氫譜表征所合成聚合單體及多肽的結(jié)構(gòu)。利用傅里葉變換衰減全反射紅外光譜（ATR�睩TIR）及X射線光電子能譜（XPS）分別表征材料表面化學(xué)結(jié)構(gòu)、元素及含量變化。通過表面接觸角測(cè)試材料的親疏水性。采用MTT法對(duì)表面接枝抗菌多肽的PPENK進(jìn)行細(xì)胞毒性測(cè)試。使用大腸桿菌及金黃色葡萄球菌對(duì)表面接枝抗菌多肽的PPENK進(jìn)行抑菌活性測(cè)試。結(jié)果表明，點(diǎn)擊化學(xué)的方法可以將多肽成功固定在PPENK表面，多肽的引入可以提高PPENK材料的親水性。細(xì)胞毒性測(cè)試表明，細(xì)胞的存活率均超過80%；抑菌活性測(cè)試說明，在PPENK表面引入陽離子抗菌多肽后，可以提高材料對(duì)細(xì)菌的抑制作用，從而達(dá)到抗菌的效果。

Abstract:

Antimicrobial functionalization is an effective way to inhibit bacterial growth on biomedical materials. Antimicrobial polypeptides have several features such as high antimicrobial activity and low cytotoxicity. Poly(arylene ether nitrile)s containing phthalazinone moieties (PPENK) is a promising polyarylether biomaterials owing to its mechanical properties and being easy to sterilize. Antimicrobial surface functionalization of PPENK with polypeptides can expand the application of poly(phthalazione aryl ether)s in biomedical field. Two kinds of polypeptides were synthesized by N�瞔arboxy angydride ring opening polymerization induced by primary amine. Then, the cationic antimicrobial polypeptides were immobilized on PPENK surface by click chemistry method. The monomer and synthesized polypeptides were characterized by NMR spectra. The samples were characterized by ATR�睩TIR, XPS measurements and contact angle. The cell viability of MC3T3�睧1 preosteoblast cells on samples were evaluated by MTT assay, and the antimicrobial activity was tested by escherichia coli and staphylococcus aureus. The results showed that the cationic antimicrobial polypeptides were successfully immobilized on PPENK surface. The viability of cells cultured with extracts from polypeptides grafted PPENKs was more than 80%. The introduction of cationic antimicrobial polypeptides on the surface of PPENK could inhibit the growth of bacteria.