文章信息/Info

Title:

3D Bioprinting with Stem Cells Based on Collagen

作者:

倪添雨; 張雅;  劉敏; 裴仁軍

中國科學院蘇州納米技術與納米仿生研究所

Author(s):

Ni Tianyu;  Zhang Yajie;  Liu Min;  Pei Renjun

Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences

關鍵詞:

膠原蛋白; 干細胞; 3D生物打印; 水凝膠; 組織工程

Keywords:

collagen;  stem sells;  3D bioprinting;  hydrogels;  tissue engineering

DOI:

10.7502/j.issn.1674-3962.201810020

文獻標志碼:

A

摘要:

干細胞3D打印是以“生物打印機”為手段，以干細胞、生長因子、生物材料等為主要內容重建人體組織或器官的跨學科領域的新型再生醫(yī)學工程技術，有著美好的發(fā)展前景和巨大的社會價值。但細胞活性的維持、打印材料和打印過程與細胞的相容、快速凝膠化等要求限制了可打印水凝膠材料的選擇。膠原蛋白是天然生物組織中細胞外基質的重要成分，其優(yōu)異的生物相容性使其在干細胞3D打印中有天然優(yōu)勢。本文綜述了基于膠原蛋白的幾種物理、離子作用及共價作用凝膠化方式，以及干細胞3D打印支架在組織工程上的研究進展。

Abstract:

The 3D bioprinting with stem cells has great prospect in organ or tissue repair. It constructs the 3D scaffolds with stem cells, growth factors and hydrogel biomaterials to regenerate the injured organs with the help of 3D bioprinter. However, the printable hydrogel biomaterials are deficient by considering the maintained activity of stem cells, biocompatibility of the materials and the printing process, fast gelation and so on. Collagen, an essential component of extracellular matrix in natural tissues, has emerged as a hydrogel material for 3D printing with great advantages in encapsulating stem cells. In this review, the gelation of collagen hydrogels in physical, ion-bonding and covalent-bonding ways and their advances in 3D bioprinting with stem cells for tissue engineering are highlighted.