文章信息/Info

Title:

Microscale tissue engineering and the bottom-up assembly for 3D tissue construction

作者:

肖文謙; 孫靜; 范紅松*

四川大學(xué)國(guó)家生物醫(yī)學(xué)材料工程技術(shù)研究中心

Author(s):

 XIAO Wenqian' target="_blank" rel="external">"sans-serif"">XIAO Wenqian;  SUN Jing and FAN Hongsong

National Engineering Research Center for Biomaterials, Sichuan University

關(guān)鍵詞:

組織工程; 微尺度組織工程; 微制造; 水凝膠; 組裝

DOI:

10.7502/j.issn.1674-3962.2015.07.13

文獻(xiàn)標(biāo)志碼:

A

摘要:

自然組織是由高度復(fù)雜化的組織微單元結(jié)構(gòu)體組成，如何在體外重現(xiàn)這些微結(jié)構(gòu)體的結(jié)構(gòu)和功能是現(xiàn)今組織工程學(xué)的巨大挑戰(zhàn)。微尺度組織工程是一項(xiàng)利用現(xiàn)代微制造技術(shù)加工和控制支架材料的微觀空間特征從而精確控制細(xì)胞微環(huán)境，在微尺度水平上引導(dǎo)和促進(jìn)制備具有模擬天然組織的非同質(zhì)性和各向異性的人造組織工程復(fù)合體的新型組織工程構(gòu)建技術(shù)。微尺度組織工程通過(guò)制備功能化微結(jié)構(gòu)單元并通過(guò)“自下而上”途徑構(gòu)建路徑使得制備出具有復(fù)雜精細(xì)結(jié)構(gòu)的工程化組織成為可能，也為組織工程提供了一條新的思路。本文對(duì)微尺度組織工程的常用技術(shù)和構(gòu)建方式做了詳細(xì)介紹，重點(diǎn)闡述了通過(guò)微凝膠單元制造和自下而上的組裝構(gòu)建方式制備組織工程復(fù)合體的新技術(shù)，并對(duì)該領(lǐng)域的未來(lái)應(yīng)用和挑戰(zhàn)進(jìn)行了展望。

Abstract:

Since most living tissues are composed of repeating micro-units with highly complex organizational structure, one of the major challenges in tissue engineering is to reproduce the architectures and functional properties of these micro-units in vitro. Microscale tissue engineering is a new emerging field which can precisely control the cellular microenvironment by the regulation of the microscopic geometry of scaffold using modern microfabrication technology. Accordingly, the simulation of natural tissues with non-homogeneous and isotropic heterosexual artificial can be directed and promoted on the microscale level. Through fabrication the micro-units and assembly by the “bottom-up” approach, construction the engineered tissue with complicated structure became possible .Therefore the mciroscale tissue engineering could provide us a new pathway and idea for the tissue engineering. In this paper, an overview of microfabrication technologies and construction approaches common used in microscale tissue engineering were presented, and the future applications and challenges of this field were discussed. Meanwhile, a bottom-up approach to direct the assembly of cell-laden microgels to generate tissue constructs was illustrated.