文章信息/Info

Title:

Recombinant fusion protein based biomaterials for tissue engineering

作者:

張妍 許可 高超 楊軍*

南開(kāi)大學(xué)生命科學(xué)學(xué)院生物活性材料教育部重點(diǎn)實(shí)驗(yàn)室,天津300071

Author(s):

ZHANG Yan;  XU Ke;  GAO Chao;  YANG Jun*

State Key Laboratory of Medicinal Chemical Biology, College of Life Science, Nankai University, Tianjin 200071, China

關(guān)鍵詞:

融合蛋白; 組織工程; 細(xì)胞外基質(zhì); 細(xì)胞外微環(huán)境; 血管化; 干細(xì)胞

DOI:

10.7502/j.issn.1674-3962.2015.03.03

文獻(xiàn)標(biāo)志碼:

A

摘要:

仿生生物體組織的結(jié)構(gòu)與狀態(tài)，研制細(xì)胞與支架材料三維復(fù)合體，將有望開(kāi)發(fā)出用于修復(fù)受損組織和器官的生物替代品。細(xì)胞的正常生物活動(dòng)需要來(lái)自細(xì)胞外微環(huán)境中的信號(hào)分子持續(xù)刺激。作為人工細(xì)胞外基質(zhì)的重要組成部分，材料的物理及化學(xué)信號(hào)能夠影響細(xì)胞基因及蛋白表達(dá)，但遠(yuǎn)不足以達(dá)到體外重建組織器官的長(zhǎng)遠(yuǎn)目標(biāo)。利用基因與蛋白質(zhì)工程技術(shù)對(duì)天然蛋白結(jié)構(gòu)進(jìn)行融合改造，設(shè)計(jì)并生物合成具有多重結(jié)構(gòu)與功能的融合蛋白生物材料,可用于改善天然或化學(xué)合成生物材料的生物活性。本文綜述了國(guó)內(nèi)外基于融合蛋白的生物材料的發(fā)展動(dòng)態(tài)，概括了融合蛋白的設(shè)計(jì)思路與制備技術(shù)，并重點(diǎn)詳述了細(xì)胞生長(zhǎng)因子以及細(xì)胞黏附因子相關(guān)融合蛋白在組織工程領(lǐng)域的應(yīng)用。

Abstract:

The construction of 3D biological substitutes consisting of cells and scaffolds is an expected approach for tissue and organ restoration. Normal biological activities of cells requires the continuous stimulation of signaling molecules from the extracellular microenvironment. As an important part of the artificial extracellular matrix, both chemical signals and physical forces of the biomaterials remodeling regulate cell gene and protein express but far not enough to sculpt tissue structure. Using gene and protein engineering technology, a fusion protein could be designed with multiple functions and improved the biological activities of natural or synthetic materials. In this article, we summarize the developments of biomaterials based on the fusion protein, as well as the concepts of fusion protein design and preparation. Meanwhile, we highlight the applications of fusion proteins that related to cell growth factors and cell adhesion factors in tissue engineering.